Technical Data

314569
Specificity
Human Mouse
Source Antibody
human
Purity
Purified by immunoaffinity chromatography.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
-20°C
eIF2b(phosphorylated Ser2)
EIF2S2, EIF2, EIF2B

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Structure of the beta subunit of translational initiation factor eIF-2. The DNA sequence and comparative analysis of human chromosome 20. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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