352698
Gene ID
176Kit Type
Sandwich ELISATests
96Sample Volume
50-100ulSensitivity
<50pg/mlDetection Method
ColorimetricDetection Range
1.56-100ng/mlSample Matrix
Cell culture supernates, serum and urineEU Commodity Code
38220000Shipping Temp
Blue IceStorage Temp
4°C/-20°CAggrecan, BioAssay™ ELISA Kit (Human)
Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.
Sample Type
Cell culture supernates, serum and urine
Intended Use
For quantitative detection of human Aggrecan in cell culture supernates, serum and urine.
Specificity
Natural and recombinant human Aggrecan
Test Principle
This human Aggrecan BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Aggrecan has been precoated onto 96-well plates. Standards (NSO) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Aggrecan is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Aggrecan amount of sample captured in plate.
Kit Components
96-well plate precoated with human Aggrecan antibody, 1 Lyophilized recombinant human Aggrecan standard, 100ng/tube×2 Biotinylated human Aggrecan antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.