HRP Conjugated, IgG SABC (Streptavidin-Biotin Complex) Detection Kit (Mouse/Rabbit)
Kit Components
1. Normal goat serum blocking reagent: 10x, 10ml, for the block of tissue sections. 2. Biotinylated Secondary Antibody (Goat Anti-mouse IgG and Goat Anti-rabbit IgG): 100~200x, 1ml (2mg/ml) each. Affinity purified antibody, labeled with “long-arm” biotin (Biotinamidohexanoic acid N-hydroxysuccinimide ester, CAS# 72040-63-2). 3. SABC-POD (HRP conjugated streptavidin): 100~200x, 1ml (2mg/ml). 4. Three drop bottles (For dilution use).
Applications
Suitable for use in Immunohistochemistry (paraffin), Immunohistochemistry (frozen), Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (paraffin): 1:50-200 Immunohistochemistry (frozen): 1:50-200 Immunocytochemistry: 1:50-200 Optimal dilutions to be determined by the researcher.
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
