353018
Gene ID
19773Kit Type
Sandwich ELISATests
96Sample Volume
50-100ulSensitivity
<10pg/mlDetection Method
ColorimetricDetection Range
78-5000pg/mlSample Matrix
Cell culture supernates, serum and plasma (heparin)EU Commodity Code
38220000Shipping Temp
Blue IceStorage Temp
4°C/-20°CRelaxin 1, BioAssay™ ELISA Kit (Mouse)
Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to mouse chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix(cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.
Sample Type
Cell culture supernates, serum and plasma (heparin)
Intended Use
For quantitative detection of mouse Relaxin 1 in cell culture supernates, serum and plasma (heparin).
Specificity
Natural and recombinant mouse Relaxin 1
Test Principle
This mouse Relaxin 1 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Relaxin 1 has been precoated onto 96-well plates. Standards(E.coli, (B) & (A)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Relaxin 1 amount of sample captured in plate.
Kit Components
96-well plate precoated with mouse Relaxin 1 antibody, 1 Lyophilized recombinant mouse Relaxin 1 standard, 10ng/tube×2 Biotinylated mouse Relaxin 1 antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
