353085
Gene ID
4804Kit Type
Sandwich ELISATests
96Sample Volume
50-100ulSensitivity
<10pg/mlDetection Method
ColorimetricDetection Range
62.5-4000pg/mlSample Matrix
Cell culture supernates, serum and plasma (heparin, EDTA, citrate).EU Commodity Code
38220000Shipping Temp
Blue IceStorage Temp
4°C/-20°CTNFRSF16/NGFR, BioAssay™ ELISA Kit (Human) (Nerve Growth Factor Receptor)
Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. The nerve growth factor receptor gene is at human chromosome region 17q12-17q22, distal to the chromosome 17 breakpoint in acute leukemias. The neurotrophin receptor p75(NTR), a tumor necrosis factor receptor superfamily member expressed in hepatic stellate cells after fibrotic and cirrhotic liver injury in humans, is a regulator of liver repair. In mice, depletion of p75(NTR) exacerbated liver pathology and inhibited hepatocyte proliferation in vivo. In addition, it is showed that neurotrophins activate p75(NTR) to induce apoptosis through the induction of the sphingomyelin (SM) cycle and increased production of ceramide. Overexpression of p75(NTR) is also found to activate the SM pathway.
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA, citrate).
Intended Use
For quantitative detection of human TNFRSF16 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Specificity
Natural and recombinant human TNFRSF16
Test Principle
This human TNFRSF16 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF16 has been precoated onto 96-well plates. Standards (sf21) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF16 amount of sample captured in plate.
Kit Components
96-well plate precoated with human TNFRSF16 antibody, 1 Lyophilized recombinant human TNFRSF16 standard, 10ng/tube×2 Biotinylated human TNFRSF16 antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
