Technical Data

396582
Kit Type
Sandwich ELISA
Tests
96
Sample Volume
50-100ul
Sensitivity
<10pg/ml
Detection Method
Colorimetric
Detection Range
156pg/ml-10,000g/ml
Sample Matrix
Cell culture supernates, serum, bone tissue, tissue.
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C
BMP-5 BioAssay™ ELISA Kit (Bone Morphogenetic Protein 5) (Monkey)

Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's, BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on monkey chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18kD.

Sample Type
Cell culture supernates, serum, bone tissue, tissue.
Intended Use
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Monkey BMP-5.
Sensitivity
<10pg/ml
Range
156pg/ml-10,000g/ml
Specificity
Recognizes natural and recombinant monkey BMP-5. There is no detectable cross-reactivity with other relevant proteins.
Test Principle
This Monkey BMP-5 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Monkey BMP-5 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A317-H454) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Monkey BMP-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 215 Monkey BMP-5 amount of sample captured in plate.
Kit Components
96-well plate precoated with anti-Monkey BMP-5 antibody, 1x plate Lyophilized recombinant Monkey BMP-5 standard, 2x10ng/tube Biotinylated anti-Monkey BMP-5 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hahn GV, Cohen RB, Wozney JM, Levitz CL, Shore EM, Zasloff MA, Kaplan FS (Dec 1992). "A bone morphogenetic protein subfamily: chromosomal localization of human genes for BMP5, BMP6, and BMP7". Genomics 14 (3): 759–62. |2. Beck HN, Drahushuk K, Jacoby DB, Higgins D, Lein PJ (Mar 2003). "Bone morphogenetic protein-5 (BMP-5) promotes dendritic growth in cultured sympathetic neurons". BMC Neurosci 2: 12.|3. "Entrez Gene: BMP5 bone morphogenetic protein 5". |4. Hahn, G. V., Cohen, R. B., Wozney, J. M., Levitz, C. L., Shore, E. M., Zasloff, M. A., Kaplan, F. S.A bone morphogenetic protein subfamily: chromosomal localization of human genes for BMP5, BMP6, and BMP7.Genomics14: 759-762, 1992.
USBio References
No references available
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