Tissue homogenates, cell lysates and other biological fluids

The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BMPER in rat tissue homogenates, cell lysates and other biological fluids.

0.058ng/ml

0.156-10ng/ml

This assay has high sensitivity and excellent specificity for detection of BMP Binding Endothelial Regulator (BMPER). No significant cross-reactivity or interference between BMP Binding Endothelial Regulator (BMPER) and analogues was observed.

Intra-Assay: CV<10% Inter-Assay: CV<12%

The microtiter plate provided in this kit has been pre-coated with an antibody specific to BMPER. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to BMPER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BMPER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BMPER in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96-well strip plate, 1x Plate Standard, 2x vial Detection Reagent A, 1×120ul Detection Reagent B, 1×120ul TMB Substrate, 1×9ml Wash Buffer (30X), 1×20ml Standard Diluent, 1×20ml Assay Diluent A, 1×12ml Assay Diluent B, 1×12ml Stop Solution, 1x6ml

Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.