9.375pg/ml

15.6-1000pg/ml

This assay has high sensitivity and excellent specificity for detection of human IL1F10. No significant cross-reactivity or interference between IL1F10 and analogues was observed.

Intra-Assay: CV<8% Inter-Assay: CV<10%

This ELISA kit employs the sandwich enzyme-linked immune-sorbent assay technology. Anti-IL1F10 antibody was pre-coated onto 96-well plates. The biotin conjugated anti-IL1F10 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL1F10 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL1F10 can be calculated.

Micro ELISA Plate(Dismountable), 8×12 Lyophilized Standard, 2x vial Sample / Standard dilution buffer, 1x20ml Biotin-labeled antibody (Concentrated), 1x120ul Antibody dilution buffer, 1x10ml HRP-Streptavidin Conjugate(SABC), 1x120ul SABC dilution buffer, 1x10ml TMB substrate, 1x10ml Stop solution, 1x10ml Wash buffer (25X), 1x30ml Plate Sealer, 1x5pieces

Store unopened kit at 4ºC; store at -20ºC once opened. Store unopened standard at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.