0.094ng/ml

0.156-10ng/ml

This assay has high sensitivity and excellent specificity for detection of human C1orf12/ECYT3/ Egl nine homolog 1/ EGLN1, HIF PH2, HIF prolyl hydroxylase 2/ HIFPH2/ HPH 2/ HPH2/PHD2/ PNAS 137/ SM 20/ SM20/ZMYND6 . No significant cross-reactivity or interference between C1orf12/ECYT3/ Egl nine homolog 1/ EGLN1, HIF PH2, HIF prolyl hydroxylase 2/ HIFPH2/ HPH 2/ HPH2/PHD2/ PNAS 137/ SM 20/ SM20/ZMYND6 and analogues was observed.

Intra-Assay: CV<8% Inter-Assay: CV<10%

This ELISA kit employs the sandwich enzyme-linked immune-sorbent assay technology. Anti-C1orf12/ECYT3/ Egl nine homolog 1/ EGLN1, HIF PH2, HIF prolyl hydroxylase 2/ HIFPH2/ HPH 2/ HPH2/PHD2/ PNAS 137/ SM 20/ SM20/ZMYND6 antibody was pre-coated onto 96-well plates. The biotin conjugated anti-C1orf12/ECYT3/ Egl nine homolog 1/ EGLN1, HIF PH2, HIF prolyl hydroxylase 2/ HIFPH2/ HPH 2/ HPH2/PHD2/ PNAS 137/ SM 20/ SM20/ZMYND6 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the C1orf12/ECYT3/ Egl nine homolog 1/ EGLN1, HIF PH2, HIF prolyl hydroxylase 2/ HIFPH2/ HPH 2/ HPH2/PHD2/ PNAS 137/ SM 20/ SM20/ZMYND6 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of C1orf12/ECYT3/ Egl nine homolog 1/ EGLN1, HIF PH2, HIF prolyl hydroxylase 2/ HIFPH2/ HPH 2/ HPH2/PHD2/ PNAS 137/ SM 20/ SM20/ZMYND6 can be calculated.

Micro ELISA Plate(Dismountable), 8×12 Lyophilized Standard, 2x vial Sample / Standard dilution buffer, 1x20ml Biotin-labeled antibody (Concentrated), 1x120ul Antibody dilution buffer, 1x10ml HRP-Streptavidin Conjugate(SABC), 1x120ul SABC dilution buffer, 1x10ml TMB substrate, 1x10ml Stop solution, 1x10ml Wash buffer (25X), 1x30ml Plate Sealer, 1x5pieces

Store unopened kit at 4ºC; store at -20ºC once opened. Store unopened standard at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.