78.13-5000pg/ml

46.88pg/ml

Intra-Assay %CV: <10% Inter-Assay %CV: <10%

Recognizes human pNF-H. No significant cross-reactivity or interference between human pNF-H and analogues was observed.

The microtiter strips provided in this kit has been pre-coated with an antibody specific to pNF-H. Standards or samples are then added to the appropriate microtiter strip wells with a biotin-conjugated antibody specific to pNF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Free components are washed away. The substrate solution is added, only those wells that contain pNF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will apear blue in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm ± 2nm. The OD value is proportional to the concentration of human pNF-H. The concentration of pNF-H in the sample is then determined by comparing the O.D. of the sample to the standard curve.

*530048A: Microtiter Strips, 1x96 wells, Pre-coated; ready to use *530048B: Standard, 2x1vial 530048C: Standard Diluent, 1x20ml *530048D: Biotin Detection, 100X, 1x120ul *530048E: HRP Conjugate, 100X, 1x120ul 530048F: Biotin Detection Diluent, 1x14ml 530048G: HRP Diluent, 1x14ml 530048H: Substrate, 1x10ml 530048K: Stop Solution, 1x10ml 530048L: Wash Buffer, 25X, 1x30ml

Store *530048A, *530048B, *530048D and *530048E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 90 minutes at 37°C. 3. Aspirate and add 100ul prepared Biotin Working Solution. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared HRP Detection Working Solution. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate. Incubate 15 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.