Technical Data

C2403-09-ML550
Conjugate
MaxLight™550
Specificity
Recognizes the human 45kD leukocyte surface glycoprotein specific for the CD48 antigen which is closely related to the activation antigen, Blast-1. It is useful in the detection of CD48 positive cells and may be used in studies to differentiate haemapoietic tumors, sarcomas and melanomas. |Species Crossreactivity: Rhesus monkey
Source Antibody
human
Purity
Purified by Protein A affinity chromatography.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
CD48 (MaxLight 550)

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo
Recommended IHC Positive Control
Tonsil
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Korinek V. et al. (1991). Immunogenetics. 33: 108-112. 2. Cinek, T. and Horejsi, V. (1992). J. Immunol. 149: 2262.
USBio References
No references available
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