Green Fluorescent Protein, Recombinant (GFP)
GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications.
Full -length recombinant protein corresponding to Green Fluorescent Protein with N-terminal His-tag, expressed in E. coli. Accession: AAA27722.
Applications
Suitable for use as a standard for protein gels and Western Blots (~28kD). Also suitable for calibration of fluorometers and microinjection into cells and tissues. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
For long-term storage, store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
Purified using Ni-NTA agarose (~70%).
Form
Supplied as a liquid in PBS, 20% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
