I1890-32A-ML550
Conjugate
MaxLight™550Specificity
Recognizes human secretory-piece and secretory IgA. No reaction against myeloma IgA (native or reduced and alkylated). Negative against normal human IgM, myeloma IgM, normal human IgG; free kappa or gamma chains and J-chain. Typical pattern on frozen and paraffin embedded sections of intestinal and breast tissue.Source Antibody
humanPurity
Purified by Protein A affinity chromatography from ascites.EU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
IgA, Secretory Component (MaxLight 550)
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
IgA is the most abundant immunoglobulin in body fluids and the second most abundant immunoglobulin in plasma, found at a concentration of 0.4 to 2.2mg/ml. It plays a very important role in the first specific defense against natural infection. Secretory IgA differs from serum IgA in that it contains two additional peptides: the secretory component and the J chain.
Applications
Suitable for use in Immunohistochemistry and FLISA. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Frozen and Paraffin sections Optimal working dilutions to be determined by researcher.
Hybridoma
NS1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
