I1904-83-ML550
Conjugate
MaxLight™550Specificity
Recognizes human IgG2 (kappa and Lambda light chain). No crossreactivty was found against IgG1, IgG3, IgG4, IgM, IgA and IgD.Source Antibody
humanPurity
Purified by Protein A affinity chromatography.EU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
IgG2, Fc (MaxLight 550)
MaxLight™ 550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains-two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG constitutes 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells. There are 4 IgG isotypes, IgG1, IgG2, IgG3 and IgG4. IgG2 makes up 23% of total IgG.
Applications
Suitable for use in FLISA, Western Blot and Affinity Chromatography. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
