This ELISA assay is a 3.5 hour solid phase immunoassay readily applicable to measure the levels of eight cytokines in cell culture supernatant, and other biological fluids. It showed no cross reactivity with various other proteins. This Multiplex ELISA is expected to be effectively used for investigations into the relationship inflammatory cytokine expression and various experimental, pathological and physiological conditions.

I8432-08A: MICROTITER Strips, 1x96 wells I8432-08B: Biotin Conjugate Mixture, 1x6ml. Mixture of Biotin conjugated anti-human cytokine antibodies I8432-08C: HRP Conjugate Mixture, 1x11ml. Mixture of Horseradish Peroxidase Conjugates *I8432-08D: STANDARD, 2x1vial, Two vials of lyophilized standard mixture. Each vial contains a buffered protein base and eight pro-inflammatory cytokines at different concentrations: IL-1α 1600pg, IL1β 2000pg, IL-6 700pg, IL-8 2000pg, GM-CSF 1800pg, IFN-γ 1300pg, MCAF 1600pg, TNF-α 1800pg. I8432-08E: CALIBRATOR DILUENT I, 1x25ml. PBS buffer containing BSA. For serum/plasma testing and preservative. I8432-08F: CALIBRATOR DILUENT II, 1x25ml. Cell culture medium RPMI 1640 with newborn calf serum and preservative. For cell culture supernatant testing. I8432-08G: WASH BUFFER, 20X, 1x60ml. 20-fold concentrated solution of buffered surfactant. I8432-08H: SUBSTRATE A, 1x10ml. Buffered solution with H2O2 I8432-08J: SUBSTRATE B, 1x10ml. Buffered solution with acetone, TMB. I8432-08K: Stop Solution, 2N H2SO4, 1x14ml (Caution: Caustic Material!)

Store *I8432-08D powder at 4°C liquid at -70°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.