M3398-55M-ML550
Conjugate
MaxLight™550Specificity
Recognizes the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. Specific to the methylated base and has|minimal reactivity to non-methylated cytidine or cytosine. Species Crossreactivity: human and mouse.Source Antibody
humanPurity
Purified by Protein A affinity chromatography.EU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
5-Methylcytidine (MaxLight 550)
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Applications
Suitable for use in Dot Blot, Immunofluorescence, Flow Cytometry, Western Blot, Methylated DNA Immunoprecipitation, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 10ul labels 10e6 cells in 100ul Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
