Technical Data

P4186-27N
Specificity
Recognizes human PI4KII-M1.
Source Antibody
human
Purity
Purified by ammonium sulfate precipitation.
Accession Number
NP_060895
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
-20°C
PI4K2A, NT (Phosphatidylinositol 4-kinase Type 2 alpha, PIK42A, Phosphatidylinositol 4-kinase Type II-alpha, PI4KII, DKFZp761G1923, RP11-548K23.6)
Phosphatidylinositol 4-kinase Type 2-alpha, EC=2.7.1.67

PI4K2A, together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3).

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Minogue,S., J. Biol. Chem. 276(20), 16635-16640 (2001). 2. Deloukas P., Nature 429:375-381(2004).
USBio References
No references available
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