P4256-29N
EU Commodity Code
38220090Shipping Temp
Dry IceStorage Temp
-70°CPlasminogen Activator Inhibitor Type 1 mutant, vitronectin binding (PAI-1)
Plasminogen Activator Inhibitor 1 (PAI-1) is a glycoprotein found in plasma that is implicated in the processes of angiogenesis, hemostasis, tumor metastasis, fibrionlysis, ovulation, embryonic development and inflammation (1). The elevation of PAI-1 has shown to correlate with the prognosis of many cancers including breast cancer (2,3,4). PAI-1 is activated by the glycoprotein vitronectin. Vitronectin binds to PAI-1 and modulates fibrinolysis and cell migration (5). This binding has proven to be an important inhibitor of the activated form of protein C (APC) (6).
Physical Appearance
Colorless frozen solution
Solubility
>2mg/ml and <5mg/ml
Buffer Composition
0.05M sodium phosphate; 0.1M sodium chloride; 1mM EDTA; pH 6.6
Purity
>98% pure by SDS PAGE 95 (+/-) 5% active by uPA titration.
Absorbance (280nm): 3.6 E0.1% = 1.0 Concentration = 3.6mg/ml Active Concentration = 3.4mg/ml
Second order rate constants for inhibition of
UPA = 6.3 X 106 M^-1s^-1 tPA = 5.7 X 106 M^-1s^-1
Storage and Stability
Store frozen at -70°C – stability >1 year.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Minor, Kenneth H and Peterson, Cynthia B. (2002) J. Biol. Chem. 277, 10337-45. Backovic, Marija, et al. (2002) Protein Science. 11, 1182-91.|General references:|1. Sherman, P.M. et al. (1992) J Biol Chem 267:7588-7595.|2. McMahon, G. A. et al. (2001) J Biol Chem 276:33964-33968.|3. Devy, L. et al. (2002) FASEB J 16:147-154.|4. Schneider, j. et al. (2003) Brit J Canc 88(1):96-101|5. hou, A. et al. (2003) 10(7):541-544|6). Rezaie, R. et al. (2001) J Biol Chem 276:15567-15570|7. Stegansson, S. et al. (1998) J Biol Chem 273:6358-6366USBio References
No references available