P9102-47B
Conjugate
FITCPurity
Chromatographically purified Protein G. Single band by IEP against anti-Fluorescein and anti-Protein G.EU Commodity Code
38220090Shipping Temp
Blue IceStorage Temp
-20°CProtein G, Native (FITC)
G. streptococcus sp.
Native Protein G is a surface receptor from Streptococcus sp. which binds the Fc portion of immunoglobulins, binding 2 molecules of IgG.
Applications
Suitable for use in Immunomicroscopy and FLISA. Other applications not tested.
Recommended Dilutions
Immunomicroscopy: 1:500-1:2500 Optimal dilutions to be determined by the researcher.
Reconstitution
Use 1ml of ddH2O. For extended storage reconstitute product with sterile 50% glycerol, ddH2O instead of water and then aliquot contents and freeze at -20°C or lower.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Heat-inactivated protein-G-bearing group of G. streptococcus sp.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Conjugated to Fluorescein isothiocyanate (FITC) (MW 390D).
Absorption Wavelength
495nm
Fluorochrome/Protein Ratio
~5 moles FITC per mole of Protein G
Purity
Chromatographically purified Protein G. Single band by SDS-PAGE.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
