R1660-01D
Specificity
Detect endogenous levels of Rb protein only when phosphorylated at Ser780, Ser795, and Ser807/811, respectively.Purity
Purified by Protein A and peptide affinity chromatography.Gene ID
5925Kit Type
AssayTests
4Detection Method
Colorimetric/FluorescentEU Commodity Code
38220000Shipping Temp
Blue IceStorage Temp
-20°CRetinoblastom Antibody Sampler Kit, BioAssay™ (Rb)
Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
Applications
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity
Detect endogenous levels of Rb protein only when phosphorylated at Ser780, Ser795, and Ser807/811, respectively.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Sherr, C.J. (1996) Science 274, 1672-1677. 2. Nevins, J.R. et al. (1992) Science 258, 424-429. 3. Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-790. 4. Hu, Q.J. et al. (1990) EMBO J. 9, 1147-1155. 5. Knudsen, E.S. and Wang, J.Y. (1997) Mol. Cell. Biol. 17, 5771-5783. 6. Lundberg, A.S. and Weinberg, R.A. (1998) Mol. Cell. Biol. 18, 753-761. 7. Connell-Crowley, L. et al. (1997) Mol. Cell. Biol. 8, 287-301. 8. Kitagawa, M. et al. (1996) EMBO J. 15, 7060-7069. 9. Geng, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 194-199.USBio References
No references available