Technical Data

T3774-92
Kit Type
Assay
Tests
100
Detection Method
Colorimetric/Fluorescent
Detection Range
Colorimetric 1-30uM|Fluorometric 0.1-1.5uM
Sample Matrix
Serum, plasma, urine, saliva and other biological sample
EU Commodity Code
38220000
UN DOT Shipping
UN1839 PGII
Shipping Temp
RT
Storage Temp
-20°C
Thiobarbituric Acid Reactive Substances (TBARS) Colorimetric BioAssay™ Kit

Oxidative attack of essential cell components by reactive oxygen species has been associated with several human diseases, such as atherosclerosis, cardiovascular diseases, diabetes, liver disorders, and inflammatory rheumatic diseases. Thiobarbituric Acid Reactive Substances (TBARS) are low-molecular-weight end products (mainly malondialdehyde, MDA) that are formed during the decomposition of lipid peroxidation products. Increased levels of TBARS have been demonstrated in these diseases. Simple, direct and accurate assays for TBARS find wide applications in research and drug discovery. TBARS assay is based on the reaction of TBARS with thiobarbituric acid (TBA) to form a pink colored product. The color intensity at 535nm or fluorescence intensity at (lex/em=560nm/585nm) is directly proportional to TBARS concentration in the sample.

Key Features
Sensitive and accurate. Linear detection range Colorimetric Assay: 1-30uM MDA Fluorometric Assay: 0.1-1.5uM MDA
Applications
Direct Assays: Serum, plasma, urine, saliva and other biological samples Drug Discovery/Pharmacology: Effects of drugs on TBARS.
Kit Components
T3774-92A: TBA Reagent, 1x25ml T3774-92B: Trichloroacetic Acid, 10%, 1x25ml T3774-92C: 6M MDA Standard, 1x1vial
Storage and Stability
Store all reagents at -20°C. Avoid freeze/thaw cycles. Stable for 6 months after receipt.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Yagi, K. (1976). A simple fluorometric assay for lipoperoxide in blood plasma. Biochem. Res. 15: 212-216. 2. Satoh, K. (1978). Serum lipid peroxide in cerebrovascular disorder determined by a new colorimetric method. Clin. Chim. Acts 90: 37-43. 3. Okawa H. et al (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95: 351-358.
USBio References
No references available
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