T8067-50C
Specificity
Recognizes an epitope within the C-terminal region of human Toso. Species sequence homology: mouse, rat.Source Antibody
humanPurity
Purified by immunoaffinity chromatography.EU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
-20°CToso, CT (Regulator of Fas-induced Apoptosis, Fas Apoptotic Inhibitory Molecule 3, FAIM3)
Toso, also known as Fas Apoptotic Inhibitory Molecule 3 (FAIM3). Toso is expressed by lymphoid and hematopoietic cells and is up-regulated by T-cell activation. Toso expressing cells have increased resistance to Fas-, FADD-, and TNFalpha-induced apoptosis. Toso is membrane bound, and is thought to directly bind to FADD, disrupting DISC formation and thus inhibiting caspase-8 activation. Toso is a potential proto-oncogene, and could be a therapeutic target for use in the treatment of autoimmune diseases. Despite its predicted molecular weight, Toso often migrates at 60kD in SDS PAGE.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml detects a band of ~43kD in human lung tissue lysate. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
