Technical Data

031983
Grade
Affinity Purified
Purity
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity
Recognizes human APOBEC3F.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
-20°C
APOBEC3F, NT (APOBEC3F, DNA dC->dU-editing enzyme APOBEC-3F, Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F)
APOBEC3F, DNA dC->dU-editing enzyme APOBEC-3F, Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F

This protein is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control.

Applications
Suitable for use in ELISA, Western Blot, Indirect Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Indirect Flow Cytometry: 1:10-1:50 Immunohistochemistry (FFPE): 1:10-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Khatua,A.K., et.al., Virology 400 (1), 68-75 (2010) 2. Koning,F.A., et.al., J. Virol. 83 (18), 9474-9485 (2009)
USBio References
No references available
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