034087-APC
Grade
Affinity PurifiedPurity
Purified by Protein A and peptide affinity chromatography.Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).Conjugate
APCSpecificity
Recognizes human COL1A1. Species Crossreactivity: mouseEU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeCOL1A1, CT (COL1A1, Collagen alpha-1(I) chain, Alpha-1 type I collagen) (APC)
COL1A1, Collagen alpha-1(I) chain, Alpha-1 type I collagen
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish].
Applications
Suitable for use in Western Blot, Immunohistochemistry and FLISA. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.