043838
Grade
Affinity PurifiedPurity
Purified by Protein A affinity chromatography.Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.Specificity
HumanEU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
-20°CWDR48, CT (WDR48, KIAA1449, UAF1, WD repeat-containing protein 48, USP1-associated factor 1, WD repeat endosomal protein, p80)
WDR48, KIAA1449, UAF1, WD repeat-containing protein 48, USP1-associated factor 1, WD repeat endosomal protein, p80
Regulator of deubiquitinating complexes. Acts as a strong activator of USP1 by enhancing the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself. Also activates deubiquitinating activity of complexes containing USP12 and USP46, respectively. Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. In case of infection by Herpes virus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpes virus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP. In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Kee, Y., et al. J. Biol. Chem. 285(15):11252-11257(2010)|Cohn, M.A., et al. J. Biol. Chem. 284(8):5343-5351(2009)|Cote-Martin, A., et al. J. Virol. 82(3):1271-1283(2008)|Cohn, M.A., et al. Mol. Cell 28(5):786-797(2007)|Park, J., et al. J. Virol. 77(16):9041-9051(2003)USBio References
No references available