MEM Eagle, SILAC (w/o Arginine, Lysine, Leucine, Methionine, Phenol Red) (EMEM)
Ready to use sterile-filtered MEM SILAC medium deficient in Arginine, Lysine, Leucine and Methionine. Stock solutions of each of these four non-labeled amino acids are provided with each bottle of the deficient medium to permit reconstitution of the complete medium.
Appearance
Pale yellow, clear liquid
Components
207007: MEM, 1x500ml, 207007A: L-Arg, 1,149mM , 1x500ul, Add 260.3ul 207007B: L-Lys HCl, 800mM, 1x500ul, Add 250ul 207007C: L-Leu,160mM, 2x1.25ml, Add 1.239ml 207007D: L-Met, 201mM, 1x500ul, Add 250ul
Storage and Stability
Store at 4°C Opened bottles should be capped tightly and kept in a dark, low humidity environment. Stable for 3-4 months after receipt.
Ready-to-Use Liquid
The SILAC medium prepared as a ready-to-use liquid that is sterile filled in 500ml bottles. Stock solutions of each of the four non-labeled amino acids are provided with each bottle of the deficient medium to permit reconstitution of the complete medium.
| Components shown as | mg/liter |
Inorganic Salts
| Calcium Chloride•2H2O | 264.9 |
| Magnesium Sulfate | 97.67 |
| Potassium Chloride | 400.0 |
| Sodium Bicarbonate | 2200.0 |
| Sodium Chloride | 6800.0 |
| Sodium Phosphate Monobasic | 121.7 |
Amino Acids
| L-Cystine•2HCl | 31.0 |
| L-Glutamine | 292.0 |
| L-Histidine | 31.0 |
| L-Isoleucine | 52.0 |
| L-Phenylalanine | 32.0 |
| L-Threonine | 48.0 |
| L-Tryptophan | 10.0 |
| L-Tyrosine | 36.1 |
| L-Valine | 46.0 |
Vitamins
| Calcium D-Pantothenate | 1.0 |
| Choline Chloride | 1.0 |
| Folic Acid | 1.0 |
| i-Inositol | 2.0 |
| Niacinamide | 1.0 |
| Pyridoxal•HCl | 1.0 |
| Riboflavin | 0.1 |
| Thiamine•HCl | 1.0 |
