Pan B-Cells is a monoclonal antibody directed against equine Ig light chains. Characterization studies demonstrated that Pan B-Cells bound 100% of CD5-ve peripheral blood lymphocytes and recognized all equine B-cells (Lunn et al. 1998) As the antigen recognized by Pan B-Cells appears to be present on the surface of all equine B-cells it is therefore is a reagent that can be used as a pan B-cell marker for domestic horses (Breathnach et al. 2005). Specific anti equine reagents have yet to be fully characterized for the typically recognized B-cell makers such as CD19, CD20, CD21, CD22 and CD79. While testing has demonstrated the cross reactivity of some monoclonal B-cell markers raised against other species with equine B cells, such as the CD79a antibody, an overview of which may be found in the report of the second equine leucocyte antigen workshop (Lunn et al. 1998). With specificity for equine Ig light chains, Pan B-Cells may be used to detect all equine immunoglobulin classes and subclasses in FLISA applications.

Suitable for use in Flow Cytometry and FLISA. Other applications not tested.

Flow Cytometry: 1:25-1:200, use 10ul to label 1x10e6 cells in 100ul Optimal dilutions to be determined by the researcher.

X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.