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299683 Murashige and Skoog Basal Medium with Vitamins (MS Medium) (Powder)

Specifications
References
Grade
Plant Grade
EU Commodity Code
38210000
UN DOT Shipping
UN1477 PGIII
Shipping Temp
Blue Ice
Storage Temp
4°C

Contains the macro- and micronutrients, and vitamins as described by Murashige & Skoog (1962).

Appearance
White to off-white, homogenous, free flowing powder
Solubility
Colorless, clear, complete
pH
As Reported
Directions per Liter
Dissolve 4.43 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as mg/L
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride
Cobalt Chloride•6H2O 0.025
Cupric Sulfate•5H2O 0.025
Na2EDTA•2H2O37.26
Ferrous Sulfate•7H2O27.8
Magnesium Sulfate
Manganese Sulfate•H2O16.9
Molybdic Acid (Sodium Salt)•2H2O 0.25
Potassium Iodide0.83
Potassium Nitrate1900
Potassium Phosphate, Monobasic170
Zinc Sulfate•7H2O8.6
Glycine
myo-Inositol 100
Nicotinic Acid (Free Acid) 0.5
Pyridoxine•HCl 0.5
Thiamine•HCl0.1
Total:4.43g/L
References
1. Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
USBio References
No references available
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