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299685 Murashige and Skoog Basal Medium with Vitamins and Sucrose (MS Medium w/Sucrose) (Powder)

Specifications
References
Grade
Plant Grade
EU Commodity Code
38210000
Shipping Temp
Blue Ice
Storage Temp
4°C

Contains the macro- and micronutrients, and vitamins as described by Murashige & Skoog (1962). Modified to include 30g/L sucrose.

Appearance
White to off-white, homogenous, free flowing powder
Solubility
Colorless, clear, complete
pH
As Reported
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 34.43 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at 4ºC. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as mg/L
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride 332.2
Cobalt Chloride•6H2O 0.025
Cupric Sulfate•5H2O 0.025
Na2EDTA•2H2O 37.26
Ferrous Sulfate•7H2O 27.8
Glycine 2
myo-Inositol 100
Magnesium Sulfate 180.7
Manganese Sulfate•H2O 16.9
Nicotinic Acid (Free Acid) 0.5
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Pyridoxine•HCI 0.5
Molybdic Acid (Sodium Salt)•2H2O 0.25
Sucrose 30000
Thiamine•HCl 0.1
Zinc Sulfate•7H2O 8.6
Total: 34.43g/L
References
1. Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
USBio References
No references available
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