TYCSB Agar Base (Tryptone Yeast Extract Cystine w/Sucrose) w/ Bacitracin Supplement (supplied separately)
Used in the selective isolation of Streptococcus mutans. TYCSB Agar is devised by Gold et al. (1) as a selective medium for Streptococcus mutans with bacitracin and sucrose. Streptococcusmutans is facultatively anaerobic, Gram-positive coccus-shaped bacterium commonly found in the human oral cavity. It is the primary causative agent of dental cavities (2). Conditions in the oral cavity are diverse and complex, frequently changing from one extreme to another. Thus, to survive in the oral cavity, S. mutans must tolerate rapidly harsh environmental fluctuations and exposure to various antimicrobial agents to survive (3). Tryptone and yeast extract in the medium provide nutrients essential for the growth of Streptococci. Sodium sulfite, sodium acetate, disodium phosphate, and sodium bicarbonate are sources of ions that simulate metabolism.
Appearance
Cream to yellow homogeneous free flowing powder
Solubility
Light yellow, clear, complete after autoclaving
pH (Before Autoclaving)
As reported
Directions per Liter
Dissolve 250 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Directions per Liter
Suspend 250g in 1000ml distilled water. Heat to boiling to dissolve to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Aseptically add sterile rehydrated contents of 1 vial of 257224 TYCSB supplement (1x200u). Mix well and pour into sterile Petri plates.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Agar | 15.00 |
L-Cystine Dihydrochloride | 0.2125 |
Sodium Acetate | 12.0552 |
Sodium Bicarbonate | 2.00 |
Sodium Chloride | 0.10 |
Disodium Hydrogen Phosphate | 0.5295 |
Sodium Sulfite | 0.10 |
Sucrose | 200.00 |
Tryptone | 15.00 |
Yeast Extract | 5.00 |