Technical Data

A0010
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
A Broth (Powder)
Ammonium Sulfate, Potassium Phosphate Dibasic, Potassium Phosphate Monobasic, Sodium Citrate

A Broth is a minimal medium base used in the maintenance and propagation of E. coli.

Appearance
White to off-white, homogeneous, free flowing powder
Solubility (16.5g/L)
Colorless, clear, complete
pH
6.8-7.2
Directions per Liter
Dissolve 16.5 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Media Formulation
Components shown as g/liter
Ammonium Sulfate1
Potassium Phosphate Dibasic10.5
Potassium Phosphate Mono4.5
Sodium Citrate0.5
Total g/L:16.5g

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Ausubel, F. M., et al., Current Protocols in Molecular Biology, John Wiley (1992). 2. Maniatis, T., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989).
USBio References
No references available
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