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A0930 Agar, Bacteriological

Specifications
References
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT

Bacteriological agar is a highly purified extract of select Gelidium marine algae, forming part of the cell wall, that gives excellent growth characteristics used in a wide variety of microbiological applications. Agar is a phycocolloid, a complex mixture of several sugar compounds that retains a gelatinous structure at RT after boiling. Bacteriological agar is purified by successive washings to remove or extensively reduce chemical contaminants, color and salts.

After autoclaving, agar is light amber and free of particulates, solidifying into a clear, firm gel. Agar is suitable for all molecular biology applications (i.e. bacterial and phage growth, M13 and Lambda). It is superior to standard microbiology grade agars.
Source
Gelidium seaweed
Appearance
White to light tan, homogenous, free flowing powder.
Solubility
Light tan, clear, complete after autoclaving
Moisture
≤15%
Ash
≤6%
pH (1.5%, post autoclaving)
7.2 ± 1.5
Gel Strength (1.5%)
600-1200g/cm2
Gel Point (1.5%)
34-38°C
Heavy Metals
Arsenic: ≤0.0003% Cadmium: ≤0.0001% Lead: ≤0.004% Mercury: ≤0.0001% Total Heavy Metals: ≤0.0020%
Microbiological Analysis
Salmonella: None Detected E. coli: None Detected Total Colony Count: ≤5000cfu/g Staphylococcus: None Detected
Growth Performance
E. coli: HB 101, DH 5, JM 103, HMS 174, FDA Seattle (Bacterial Control/640nm, 24/48hrs) Yeast: S. cerevisiae, K. lactis, S. pastorianus, P. pastoris, S. pombe (Yeast Control/640nm, 72 hrs)
Storage
RT
Alternate Nomenclature
Agar-agar (8CI); AX 100; AX 200; AX 200F; AX 30; Agar 150C; Agar Agar Flake; Agargel; Agaron gel; Agaropectin, mixt. with agarose; Agarose, mixt. with agaropectin; Bacto-agar; Bengal gelatin; Bengal isinglass; CS 110; CS-16A; Ceylon isinglass; Chinese isinglass; Czapeck agar; D 100; D 100 (polysaccharide); Deltagar LTS; Digenea simplex mucilage; E 406; GAM medium; Gel Up J 1630; Gel Up J 3762; Gelose; Hygicult TPC; Ina Agar CS 420; Ina Agar CS 670; Ina Agar DJ 100; Ina Agar Karikorikan; Ina Agar M 8; Ina Agar S 5; Ina Agar S 7; Ina Agar UP 37; Ina Agar UP 37CS; Ina Agar Z 10; Ina Agar ZR; Inagel N 41S; Inagel N 6; Japan agar; Japan isinglass; Kantenmatsu; LX 30; Layor Carang; Luxara 1253; M 7; MRO-FV 2000; Max; Neosoft AR; Neosoft AR 132; Oxoid III; Oxoid L 11; PS 10; PS 7; PS 96; Phytagar; S 10; S 10 (polysaccharide); S 100; S 100 (polysaccharide); S 6; S 6 (polysaccharide); S 6S; S 7; S 7 (polysaccharide); S 8; S 9; SDA; Sabouraud dextrose agar; Supunkanten SAI 7; T 1; UP 16; UP 26; UP 26K; UP 37; UP 37K; UP 6; UP 7; UX 100; UX 30; UZ 4K; Ultra Agar BX 30; Ultra Agar UX 30; Ultra-Agar; Ultra-Agar AX 100; Ultra-Agar AX 100CS; Ultra-Agar AX 2; Ultra-Agar AX 200; Ultra-Agar AX 200K; Ultra-Agar AX 30; Ultra-Agar Ina; Ultra-Agar RB; XG 89; YM 70
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
All products should be handled by qualified personnel only, trained in laboratory procedures.
References
US Biological application references: 1. Azab, A.N. et al., (2009) Life Sci. 84:38-44. 2. Azab, A.N. et al., (2007) Mol Microbiol. 63:1248-1258. 3. Madsen, J. A., et al., (2012) Chem. Eur. J.. doi: 10.1002/chem.201103534. 4. Devkota, A.K. et al., (2012) Biochemistry, 51; 2100-2112. 5. Madsen, J. A., et al., (2012) Chem. Eur. J.. doi: 10.1002/chem.201103534. 6. Wang, Liang-Chun, et al. Immunofluorescence Analysis of Human Endocervical Tissue Explants Infected with Neisseria gonorrhoeae. 2018. Bio Protoc. 8(3):e2720. doi: 10.21769/BioProtoc.2720.|General References: 1. Falk, B. & Simmons, J., Bacteriology 37: 121 (1939). 2. Hitchens, Leikind, J. Bacteriology, 37: 485 (1939). 3. Seip, In Fogg and Jones (ed), Proc. 8th Intl. Seaweed Symp. (1981). 4. United States Pharmacopeia, 23: 1721,1897 (1990).
USBio References
US Biological application references: 1. Azab, A.N. et al., (2009) Life Sci. 84:38-44. 2. Azab, A.N. et al., (2007) Mol Microbiol. 63:1248-1258. 3. Madsen, J. A., et al., (2012) Chem. Eur. J.. doi: 10.1002/chem.201103534. 4. Devkota, A.K. et al., (2012) Biochemistry, 51; 2100–2112. 5. Madsen, J. A., et al., (2012) Chem. Eur. J.. doi: 10.1002/chem.201103534. 6. Wang, Liang-Chun, et al. Immunofluorescence Analysis of Human Endocervical Tissue Explants Infected with Neisseria gonorrhoeae. 2018. Bio Protoc. 8(3):e2720. doi: 10.21769/BioProtoc.2720.
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