Technical Data

A1372-40
Grade
Microbiological Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Amies Broth with Charcoal (Amies Transport Medium) (Powder)

Amies formulated a medium for transport of microorganisms using a balanced salt solution. Amies Broth with Charcoal is a non-nutritional, phosphate buffered type medium used to maintain the viability of microorganisms without a significant increase in growth. Charcoal was added to the formulation to neutralize the toxic effects of a fatty acids that are toxic to microorganisms. This medium is recommended for throat, vaginal, and wound samples.

Appearance
Black, homogeneous, free flowing powder
Solubility
Black, opaque, semi-solid
pH
As Reported
Directions per Liter
Dissolve 18.05 grams in 800-900ml of ddH2O stirring with gently heat and bring to boiling. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/liter
Calcium Chloride 0.1
Charcoal10.0
Magnesium Chloride 0.1
Potassium Chloride 0.2
Monopotassium Phosphate 2.5
Sodium Chloride 3.0
Disodium Phosphate 1.15
Sodium Thioglycollate 1.0

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Amies C.R. (1967) "A Modified Formula for the Preparation of Stuart’s Transport Medium". Can. J. Public Health 58: 296-300.
USBio References
No references available
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