B0101-L
Grade
Cell Culture GradeForm
Light pink to orange, clear, complete liquidShipping Temp
Blue IceStorage Temp
4°CBasal Medium Eagle (BME) w/Earle’s, L-Glutamine (Liquid)
Basal Medium Eagle (BME) developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. BME was developed a result of numerous studies carried out in the late 1950’s to determine the essential nutritional requirements and other factors critical to the growth of cells in culture. BME, when properly supplemented, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines. BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME). BME is suitable for use with diploid and primary mammalian cell cultures.
Storage and Stability
Store at 4°C. Stable for 6 months after receipt.
Components shown as g/liter for a 1X Solution |
Inorganic Salts
Calcium Chloride•2H2O | 0.265 |
Magnesium Sulfate | 0.09767 |
Potassium Chloride | 0.4 |
Sodium Chloride | 6.8 |
Sodium Phosphate Mono | 0.122 |
Amino Acids
L-Arginine•HCl | 0.021 |
L-Cystine•2HCl | 0.01565 |
L-Glutamine | 0.292 |
L-Histidine | 0.008 |
L-Isoleucine | 0.026 |
L-Lysine•HCl | 0.03647 |
L-Methionine | 0.0075 |
L-Phenylalanine | 0.0165 |
L-Threonine | 0.024 |
L-Tryptophan | 0.004 |
L-Tyrosine•2Na•2H2O | 0.02595 |
L-Valine | 0.0235 |
Vitamins
D-Biotin | 0.001 |
Choline Chloride | 0.001 |
Folic Acid | 0.001 |
myo-Inositol | 0.002 |
Niacinamide | 0.001 |
D-Pantothenic Acid•Ca | 0.001 |
Pyridoxal•HCl | 0.001 |
Riboflavin | 0.0001 |
Thiamine•HCl | 0.001 |
Other
D-Glucose | 1 |
Phenol Red, Sodium | 0.011 |
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Eagle, H., et al., J. Biol. Chem. 214: 845-847 (1956). 2. Eagle, H., Tissue Culture Association Manual 3: 517-520 (1976). 3. Eagle, H., Science 130: 432-437 (1959). 4. Eagle, H., Science 122: 501 (1955).USBio References
No references available