BCYE (Powder)
Legionella Agar Base
Primary isolation media for Legionella sp.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Appearance
Gray to black, homogeneous, free flowing powder
Solubility
Gray-black to bluish-black, opalescent after autoclaving
pH (after autoclaving)
As Reported
Directions per Liter
Dissolve 37.75g in 900ml of ddH2O stirring gently. Adjust pH to 6.9 with 1N KOH. Add additional water to bring the solution to1L Heat to boiling with stirring to dissolve. Autoclave at 121°C for 15 minutes. Cool to 45-50°C. Aseptically add 10ml sterile solution of L-Cysteine (4%). Mix and add inhibitor solutions if required. Dispense with agitation.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Components shown as | g/Liter |
| ACES Buffer | 10 |
| Activated Charcoal | 1.5 |
| Agar 15 |
| Ferric Pyrophosphate | 0.25 |
| alpha-Ketoglutarate | 1.0 |
| Yeast Extract | 10 |
