BGJb Medium Fitton-Jackson Modification w/L-Glutamine (Powder)
Medium BGJ was originally developed by Biggers, Gwatkin and Judah in the early 1960's at the Wistar Institute. Subsequent studies resulted in a modification designated BGJb which has been used for supporting cultures of cartilaginous embryonic bone. An additional modification, developed by Sylvia Fitton-Jackson at Strangeways Laboratory in England, is further enriched over the prior formulae. Additional amino acids and vitamins, and increased buffering capacity conferred by the phosphates in the Fitton-Jackson modification, create conditions that permit calcification and growth of cartilaginous embryonic bone.
Appearance
Pale yellow to pale orange, homogeneous, free flowing powder
Solubility
Yellow to orange, clear, complete
Directions per Liter
Dissolve 21.3g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 3.5g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Components shown as g/liter |
Inorganic Salts
| Magnesium Sulfate | 0.09768 |
| Potassium Chloride | 0.4 |
| Sodium Acetate | 0.050 |
| Sodium Chloride | 6.8 |
| Sodium Phosphate Dibasic | 0.112 |
| Sodium Phosphate Mono | 0.02434 |
Amino Acids
| L-Alanine | 0.25 |
| L-Arginine | 0.175 |
| L-Aspartic Acid | 0.15 |
| L-Cysteine•HCL•H2O | 0.1003 |
| L-Glutamine | 0.2 |
| Glycine | 0.8 |
| L-Histidine | 0.15 |
| L-Isoleucine | 0.03 |
| L-Leucine | 0.05 |
| L-Lysine•HCl | 0.24 |
| L-Methionine | 0.05 |
| L-Phenylalanine | 0.05 |
| L-Proline | 0.4 |
| L-Serine | 0.2 |
| L-Threonine | 0.075 |
| L-Tryptophan | 0.04 |
| L-Tyrosine•2Na•2H2O | 0.05766 |
| DL-Valine | 0.065 |
Vitamins
| PABA | 0.002 |
| Ascorbic Acid, Sodium | 0.05 |
| D-Biotin | 0.0002 |
| Choline Bitartrate | 0.0907 |
| Vitamin B-12 | 0.00004 |
| Folic Acid | 0.0002 |
| myo-Inositol | 0.0002 |
| Nicotinic Acid | 0.02 |
| D-PantothenicAcid•Ca | 0.0002 |
| Pyridoxal-5-Phosphate | 0.0002 |
| Riboflavin | 0.0002 |
| Thiamine•HCl | 0.004 |
| DL-α-Tocopherol Phosphate•2Na | 0.001 |
Other
| Calcium Lactate | 0.555 |
| D-Glucose | 10 |
| Phenol Red, Sodium | 0.02 |
