Casman Medium Base (Powder)
When supplemented with blood will support growth of many fastidious bacteria including Gardnerella vaginalis.
Appearance
Beige, homogeneous, free flowing powder
Solubility
Light amber to medium amber, trace to slight haze
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Solubility (Blood)
Red, opaque
Cultural Response
Haemophilus influenza ATCC 35056 Neisseria gonorrhoeae ATCC 43070 Neisseria meningitidis ATCC 13090 Streptococcus pneumoniae ATCC 6305 Streptococcus pyogenes ATCC 19615
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions
Dissolve 43g in 1L of ddH2O. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Autoclave at 121ºC for 15 minutes. After cooling medium to 50ºC, aseptically add 5% sterile blood and 0.15% sterile water-lysed blood solution (one part blood to three parts water).
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Enzymatic Digest of Casein | 5 |
| Enzymatic Digest of Animal Tissue | 5 |
| Yeast Enriched Peptone | 10 |
| Beef Extract | 3 |
| Niacinamide | 0.05 |
| p-Aminobenzoic Acid | 0.05 |
| Dextrose | 0.5 |
| Corn Starch | 1 |
| Sodium Chloride | 5 |
| Agar | 13.5 |
