C2394-35A1
Grade
Affinity PurifiedPurity
Purified by Protein G affinity chromatography from tissue culture supernatant.Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.Specificity
Recognizes sheep CD41/CD61. Species Crossreactivity: goat, feline, rabbit, canine, equine and bovineEU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
-20°CCD41, CD61 (CD41b, GP2B, GP-IIb, GPIIb, GP3A, GP-IIIa, GPIIIa, GTA, HPA3, Integrin alpha 2b, ITGA2B, Integrin beta 3, ITGB3, Platelet Glycoprotein IIb of IIb/IIIa Complex)
CD41/CD61 is also known as GpIIb/IIIa. CD41/CD61 is a calcium-complex dependent heterodimer and is expressed almost exclusively on platelets and on their precursors, the megakaryocytes. This complex is an activation-dependent receptor for RGD (Arg-Gly-Asp)-containing glycoproteins such as fibrinogen, fibronectin, von Willebrand factor, thrombospondin and vitronectin. It plays a central role in platelet aggregation and haemostasis.
Applications
Suitable for use in ELISA, Flow Cytometry and Immunoprecipitation. Other applications have not been tested.
Recommended Dilutions
Flow Cytometry: 1:50-1:200; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Hybridoma
Sp2-0/AG14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
