Technical Data

C2555-01
Grade
Affinity Purified
Purity
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity
Recognizes the ~80kD human Cdc14A protein. Reactivity is confirmed with human HEK293 cell lysates.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
-20°C
cdc14A Phosphatase

Cdc14, also known as duel specificity phosphatase, is a protein phosphatase originally discovered in yeast that plays an important role in cell cycle regulation, including exit from mitosis. Throughout G1, S and G2 phases, Cdc14 is located in the nucleolus; at the beginning of anaphase, the protein is redistributed throughout the entire nucleus. In late anaphase, Cdc14 dephosphorylates Cdh1 which then activates APC (Anaphase Promoting Complex). Two human orthologs of yeast Cdc14 have been identified; these are Cdc14A and Cdc14B, and they share about 80% amino acid homology in the N-termini and phosphatase domains. Cdc14 has also been shown to dephosphorylate p53, suggesting that it may play a role in regulation of p53 activity.

Applications
Suitable for use in Immunofluorescence and Western Blot. Other applications have not been tested.
Recommended Dilution
Immunofluorescence: 1-5ug/ml Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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