CLED Agar (Powder)
Cystine-Lactose-Electrolyte Deficient Medium
CLED Agar (Cystine-Lactose-Electrolyte-Deficient) media was first formulated by Sandys and later modified by Mackey and Sandys. This medium supports the growth of urinary pathogens and gives good colonial differentiation of these bacteria. Lactose is the carbon source. Bromthymol blue is the indicator dye. bromthymol blue, Organisms that are lactose fermenting produce yellow colonies while non-lactose fermenting bacteria give blue colonies. Since there are no salts (electrolyte-deficient) in this media the swarming of Proteus sp. is suppressed. CLED Agar is recommended as a dip inoculum transport medium for urine specimens. A quantitative count method for urine cultures called the Drop Plate method was devised by Neblett.
Appearance
Light blue-green, homogeneous, free flowing powder
Solubility
Light greyish-green, clear, complete
Microbiological Analysis
Escherichia coli ATCC 25922: Growth Yellow Colonies Proteus mirabilis ATCC 12453: Growth Clear Colonies Salmonella typhimurium ATCC 14028 : Growth Clear Colonies Staphylococcus aureus ATCC 25923: Growth Yellow Colonies
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Litler
Mix 36.1 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Components shown as g/liter |
| Pancreatic Digest of Gelatin | 4.0 |
| Beef Extract | 3.0 |
| Casein Digest Peptone | 4.0 |
| Lactose | 10.0 |
| L-Cystine | 0.128 |
| Bromothymol Blue | 0.02 |
| Agar | 15.0 |
