Technical Data

C7905-01P-ML550
Grade
Purified
Purity
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Conjugate
MaxLight™550
Specificity
Recognizes human C-peptide. It can also be used to detect proinsulin.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
C-Peptide (MaxLight 550)

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

C-peptide also known as connecting peptide is a peptide released when proinsulin is cleaved to produce insulin.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hilgert, I. et al. (1991) A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA.Hybridoma. 10: 379-86. 2. Afrikanova, I. et al. (2011) Inhibitors of Src and Focal Adhesion Kinase Promote Endocrine Specification: Impact on the Derivation of Beta Cells from Human Pluripotent Stem Cells. J Biol Chem. Aug 18. [Epub ahead of print] 3. Vasic, D. and Walcher, D. (2012) C-Peptide: A New Mediator of Atherosclerosis in DiabetesMediators of Inflammation. Article ID: 858692.
USBio References
No references available
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