Drop-out Mix Minus Adenine, Histidine, Leucine w/o Yeast Nitrogen Base

Grade

Molecular Biology Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

Synthetic complete amino acid supplement minus adenine, histidine and leucine

Drop-out Mixes are used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants.

• Convenient Powders • Useful for One-Hybrid and Two-Hybrid Systems • Competitively Priced

Dust-Free

Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates

Homogeneous

Proprietary blending technology ensures complete mixing of components

Appearance

White, homogeneous, free flowing powder

Solubility

Colorless, clear, complete

As Reported

Directions per Liter (Broth)

Mix 1.53 grams of drop-out mix with 6.7g Y2025 Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS per liter of ddH2O. Adjust to desired pH. Sterilize with 0.2um membrane filter. Aliquot into sterile containers.

Directions per Liter (Plates)

Mix 1.53 grams of drop-out mix with 43.7g D9510-01 Drop-out Base (w/o aa) With Glucose and Agar per liter of ddH2O. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Formulation

Each bottle is a homogeneous mixture of the following amino acids and nucleotide bases minus adenine, histidine and leucine.

Amino Acids

Alanine	2.0
Arginine	2.0
Asparagine	2.0
Aspartic Acid	2.0
Cysteine	2.0
Glutamic Acid	2.0
Glutamine	2.0
Glycine	2.0
Histidine	Absent
Isoleucine	2.0
Leucine	Absent
Lysine	2.0
Methionine	2.0
Phenylalanine	2.0
Proline	2.0
Serine	2.0
Threonine	2.0
Tryptophan	2.0
Tyrosine	2.0
Valine	2.0

Nucleotide Bases

Adenine	Absent
Uracil	2.0

Vitamins

p-Aminobenzoic acid	0.2
Inositol	2.0

Use 1.53g/liter
100g makes ~50 liters

References

1. Kaiser, C., Michaelis, S., Mitchell, A., Methods in Yeast Genetics, Cold Spring Harbor Laboratory Course Manual, Appendix A: 210, CSHL Press (1994).

USBio References

1. Coradetti ST., et al. 2018. Functional genomics of lipid metabolism in the oleaginous yeast Rhodosporidium toruloides. eLife. 7: e32110. doi: 10.7554/eLife.32110|2. Czajka JJ, et al. 2018. Engineering the oleaginous yeast Yarrowia lipolytica to produce the aroma compound β-ionone. Microbial Cell Factories. 17: 136. https://doi.org/10.1186/s12934-018-0984-x.

Product	Ext Price
Subtotal:	Subtotal:
Subtotal:	Subtotal:
Total Coupon Savings:	Total Coupon Savings:	()
Your cart is currently empty.
- Coupon Code

D9515-05 Drop-out Mix Minus Adenine, Histidine, Leucine w/o Yeast Nitrogen Base (DO, Dropout, Powder)

Grade

EU Commodity Code

Shipping Temp

Storage Temp

Synthetic complete amino acid supplement minus adenine, histidine and leucine

References

USBio References

Pricing

Order

Cart Summary

Recently Viewed

Special Offers

Contact Us

Site Map

Technical Resources

Distributors

Payment Methods