Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics.
Each bottle is a homogeneous mixture of the following amino acids and nucleotide bases minus histidine and uracil. |
Alanine | 2.0g |
p-Aminobenzoic acid | 0.2g |
Arginine | 2.0g |
Asparagine | 2.0g |
Aspartic acid | 2.0g |
Cysteine | 2.0g |
Glutamic acid | 2.0g |
Glutamine | 2.0g |
Glycine | 2.0g |
Histidine | Absent |
Isoleucine | 2.0g |
Leucine | 10.0g |
Lysine | 2.0g |
Methionine | 2.0g |
Phenylalanine | 2.0g |
Proline | 2.0g |
Serine | 2.0g |
Threonine | 2.0g |
Tryptophan | 2.0g |
Tyrosine | 2.0g |
Valine | 2.0g |
Adenine | 0.118 |
Inositol | 2.0g |
Uracil | Absent |
Use 1.83g/liter | |
100g makes ~50 liters |
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