Drop-out Mix Synthetic Minus Tryptophan, Uracil w/o Yeast Nitrogen Base (DO, Dropout, Powder)
Synthetic complete amino acid supplement–minus tryptophan, uracil
Drop-out Mixes are used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants.
• Convenient Powders • Useful for One-Hybrid and Two-Hybrid Systems • Competitively Priced
Dust-Free
Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates
Homogeneous
Proprietary blending technology ensures complete mixing of components
Appearance
White, homogeneous, free flowing powder
Solubility
Colorless, clear, complete
Companion Products
D9500: Drop-out Base With Glucose (Powder) D9501: Drop-out Base With Raffinose (Powder) D9502: Drop-out Base With Galactose (Powder) D9510: Drop-out Base With Glucose and Agar (Powder) D9511: Drop-out Base With Raffinose and Agar (Powder) D9512: Drop-out Base With Galactose and Agar (Powder) Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder) Y2025: Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS (YNB) (Powder) Y2030: Yeast Nitrogen Base w/o AA, Carbohydrate & w/o AS (YNB) (Powder) Y2035: Yeast Nitrogen Base w/AA & w/AS, Vitamin Free (YNB) (Powder) Y2035-05: Yeast Nitrogen Base (YNB) w/AA & w/AS, Vitamin Free, w/o Dextrose (Powder) Y2036: Yeast Nitrogen Base w/o AA, AS, Thiamine HCl (Powder) Y2037: Yeast Nitrogen Base w/o AA & w/AS (YNB) and w/o Fe, Zn, Mn, Cu (Powder)
Directions per Liter (Broth)
Mix 1.85 grams of drop-out mix with 6.7g Y2025 Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS per liter of ddH2O. Adjust to desired pH. Sterilize with 0.2um membrane filter. Aliquot into sterile containers.
Directions per Liter (Plates)
Mix 1.85 grams of drop-out mix with 43.7g D9510-01 Drop-out Base (w/o aa) With Glucose and Agar per liter of ddH2O. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Formulation
| Each bottle is a homogeneous mixture of the following amino acids and nucleotide bases minus tryptophan, uracil. |
Amino Acids
| Alanine | 2.0g |
| p-Aminobenzoic acid | 0.2g |
| Arginine | 2.0g |
| Asparagine | 2.0g |
| Aspartic acid | 2.0g |
| Cysteine | 2.0g |
| Glutamic acid | 2.0g |
| Glutamine | 2.0g |
| Glycine | 2.0g |
| Histidine | 2.0g |
| Isoleucine | 2.0g |
| Leucine | 10.0g |
| Lysine | 2.0g |
| Methionine | 2.0g |
| Phenylalanine | 2.0g |
| Proline | 2.0g |
| Serine | 2.0g |
| Threonine | 2.0g |
| Tryptophan | Absent |
| Tyrosine | 2.0g |
| Valine | 2.0g |
Nucleotide Bases
| Adenine | 0.5g |
| Inositol | 2.0g |
| Uracil | Absent |
| Use 1.85g/liter |
| 100g makes ~50 liters |
