Drop-out Mixes are used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants.
Each bottle is a homogeneous mixture of the following amino acids and nucleotide bases minus tryptophan, uracil. |
Alanine | 2.0g |
p-Aminobenzoic acid | 0.2g |
Arginine | 2.0g |
Asparagine | 2.0g |
Aspartic acid | 2.0g |
Cysteine | 2.0g |
Glutamic acid | 2.0g |
Glutamine | 2.0g |
Glycine | 2.0g |
Histidine | 2.0g |
Isoleucine | 2.0g |
Leucine | 10.0g |
Lysine | 2.0g |
Methionine | 2.0g |
Phenylalanine | 2.0g |
Proline | 2.0g |
Serine | 2.0g |
Threonine | 2.0g |
Tryptophan | Absent |
Tyrosine | 2.0g |
Valine | 2.0g |
Adenine | 0.5g |
Inositol | 2.0g |
Uracil | Absent |
Use 1.85g/liter | |
100g makes ~50 liters |
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