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D9537-02 Drop-out Mix Synthetic Minus Tryptophan, Uracil w/o Yeast Nitrogen Base (DO, Dropout, Powder)

Specifications
References
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Synthetic complete amino acid supplement–minus tryptophan, uracil

Drop-out Mixes are used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants.

• Convenient Powders • Useful for One-Hybrid and Two-Hybrid Systems • Competitively Priced
Dust-Free
Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates
Homogeneous
Proprietary blending technology ensures complete mixing of components
Appearance
White, homogeneous, free flowing powder
Solubility
Colorless, clear, complete
pH
As Reported
Companion Products
D9500: Drop-out Base With Glucose (Powder) D9501: Drop-out Base With Raffinose (Powder) D9502: Drop-out Base With Galactose (Powder) D9510: Drop-out Base With Glucose and Agar (Powder) D9511: Drop-out Base With Raffinose and Agar (Powder) D9512: Drop-out Base With Galactose and Agar (Powder) Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder) Y2025: Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS (YNB) (Powder) Y2030: Yeast Nitrogen Base w/o AA, Carbohydrate & w/o AS (YNB) (Powder) Y2035: Yeast Nitrogen Base w/AA & w/AS, Vitamin Free (YNB) (Powder) Y2035-05: Yeast Nitrogen Base (YNB) w/AA & w/AS, Vitamin Free, w/o Dextrose (Powder) Y2036: Yeast Nitrogen Base w/o AA, AS, Thiamine HCl (Powder) Y2037: Yeast Nitrogen Base w/o AA & w/AS (YNB) and w/o Fe, Zn, Mn, Cu (Powder)
Directions per Liter (Broth)
Mix 1.85 grams of drop-out mix with 6.7g Y2025 Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS per liter of ddH2O. Adjust to desired pH. Sterilize with 0.2um membrane filter. Aliquot into sterile containers. 
Directions per Liter (Plates)
Mix 1.85 grams of drop-out mix with 43.7g D9510-01 Drop-out Base (w/o aa) With Glucose and Agar per liter of ddH2O. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Formulation
Each bottle is a homogeneous mixture of the following amino acids and nucleotide bases minus tryptophan, uracil.
Amino Acids
Alanine2.0g
p-Aminobenzoic acid0.2g
Arginine2.0g
Asparagine2.0g
Aspartic acid2.0g
Cysteine2.0g
Glutamic acid2.0g
Glutamine2.0g
Glycine2.0g
Histidine2.0g
Isoleucine2.0g
Leucine10.0g
Lysine2.0g
Methionine2.0g
Phenylalanine2.0g
Proline2.0g
Serine2.0g
Threonine2.0g
TryptophanAbsent
Tyrosine2.0g
Valine2.0g
Nucleotide Bases
Adenine0.5g
Inositol2.0g
UracilAbsent
Use 1.85g/liter
100g makes ~50 liters
References
1. Kaiser, C., Michaelis, S., Mitchell, A., Methods in Yeast Genetics, Cold Spring Harbor Laboratory Course Manual, Appendix A: 210, CSHL Press (1994).
USBio References
No references available
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