Dulbecco’s MEM (DMEM) w/o Isoleucine, Methionine, Threonine, Valine (Powder)
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.
Appearance
Light tan to orange, homogeneous, free-flowing powder
Solubility
Orange to red, clear, complete
Directions per Liter
Dissolve 11.81g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 3.7g/L sodium bicarbonate. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Components shown as | g/Liter |
Inorganic Salts
| Calcium Chloride•2H2O | 0.265 |
| Ferric Nitrate•9H2O | 0.0001 |
| Magnesium Sulfate | 0.09767 |
| Potassium Chloride | 0.400 |
| Sodium Chloride | 6.4 |
| Sodium Phosphate Monobasic | 0.109 |
Amino Acids
| L-Arginine•HCl | 0.084 |
| L-Cystine•2HCl | 0.0626 |
| L-Glutamine | 0.584 |
| Glycine | 0.030 |
| L-Histidine HCl•H2O | 0.042 |
| L-Isoleucine | Absent |
| L-Leucine | 0.105 |
| L-Lysine•HCl | 0.146 |
| L-Methionine | Absent |
| L-Phenylalanine | 0.066 |
| L-Serine | 0.042 |
| L-Threonine | Absent |
| L-Tryptophan | 0.016 |
| L-Tyrosine•2Na•2H2O | 0.10379 |
| L-Valine | Absent |
Vitamins
| Choline Chloride | 0.004 |
| Folic Acid | 0.004 |
| myo-Inositol | 0.0072 |
| Niacinamide | 0.004 |
| D-Pantothenic Acid•Ca | 0.004 |
| Pyridoxine•HCl | 0.004 |
| Riboflavin | 0.0004 |
| Thiamine•HCl | 0.004 |
Other
| D-Glucose | 3.151 |
| Phenol Red, Sodium | 0.015 |
| Pyruvic Acid, Sodium | 0.055 |
