Dulbecco’s MEM

Grade

Cell Culture Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Appearance

White, homogenous, free flowing powder.

Solubility

Colorless, clear, complete

As Reported

Endotoxin

≤1EU/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Directions per Liter

Dissolve 13.38g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as g/liter

Inorganic Salts

Calcium Chloride•2H2O	0.265
Ferric Nitrate•9H2O	0.0001
Magnesium Sulfate	0.09767
Potassium Chloride	0.4
Sodium Chloride	6.4
Sodium Phosphate Monobasic	0.109

Amino Acids

L-Arginine•HCl	0.084
L-Cystine•2HCl	0.0626
L-Glutamine	0.584
Glycine	0.03
L-Histidine•HCl•H2O	0.042
L-Isoleucine	0.105
L-Leucine	0.105
L-Lysine•HCl	0.146
L-Methionine	0.03
L-Phenylalanine	0.066
L-Serine	0.042
L-Threonine	0.095
L-Tryptophan	0.016
L-Tyrosine•Na2•2H2O	0.1038
L-Valine	0.094

Other

D-Glucose

4.5

Total:

13.38 g/L

References

1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9

USBio References

No references available

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D9801-05 Dulbecco’s MEM (DMEM) High Glucose, w/L-Glutamine, w/o Phenol Red, Na Pyruvate, Vitamins (Powder)

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