Technical Data

D9801-05A
Grade
Cell Culture Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Dulbecco’s MEM (DMEM) Vitamin Mix 25X, w/o Thiamine
Powder

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Appearance
Yellow, homogenous, free flowing powder
Solubility (1X)
Colorless to yellow, clear, complete
pH (1X)
As Reported
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 0.690g in 800-900ml of ddH2O stirring gently until completely solubilized. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/liter
Choline Chloride0.100
Folic Acid0.100
myo-Inositol0.180
Niacinamide0.100
D-Pantothenic Acid-Ca0.100
Pyroxidal HCl0.100
Riboflavin0.010
Thiamine HClAbsent
Total 0.690g/L

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Dulbecco, R. and Freeman, G. Virology. 8, 396-397. (1959). 2. Smith, J.D., Freeman,G., Vogt, M. and Dulbecco, R. The Nucleic Acid of Polyoma Virus. 12, 185-196. (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro. 6, 89. (1970). 4. Rutzky, L.P. and Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro. 9, 468. (1974).
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net