Many modifications of Eagle’s Medium have been developed since the original formulation appeared in literature. Among the most widely used of these modifications is Dulbecco’s Modified Eagle’s Medium (DMEM). DMEM is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DMEM formula contains 1000mg/L of glucose and was first reported for culturing embryonic mouse cells. A further alteration with 4500mg/L glucose has proved to be optimal in cultivating certain cell types.
Components shown as g/liter |
Calcium Chloride•2H2O | 0.265 |
Ferric Nitrate•9H2O | 0.0001 |
Magnesium Sulfate, Anhy | 0.09767 |
Potassium Chloride | 0.400 |
Sodium Chloride | 6.400 |
Sodium Phosphate Monobasic | 0.109 |
L-Arginine•HCl | 0.084 |
L-Cystine•2HCl | 0.0626 |
L-Glutamine | 0.584 |
Glycine | 0.030 |
L-Histidine•HCl•H2O | 0.042 |
L-Isoleucine | 0.105 |
L-Leucine | 0.105 |
L-Lysine•HCl | 0.146 |
L-Methionine | 0.030 |
L-Phenylalanine | 0.066 |
L-Serine | 0.042 |
L-Threonine | 0.095 |
L-Tryptophan | 0.016 |
L-Tyrosine•2Na•2H2O | 0.10379 |
L-Valine | 0.094 |
Choline Chloride | 0.004 |
Folic Acid | 0.004 |
myo-Inositol | 0.0072 |
Niacinamide | 0.004 |
D-Pantothenic Acid•Ca | 0.004 |
Pyridoxal•HCl | 0.004 |
Riboflavin | 0.0004 |
Thiamine•HCl | 0.004 |
D-Glucose | 4.500 |
Pyruvic Acid, Sodium | 0.110 |
Phenol Red, Sodium | 0.0159 |
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.