Dulbecco’s MEM

Grade

Cell Culture Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Appearance

Light orange to orange, homogenous, free flowing powder

Solubility

Light pink to orange, clear, complete

As Reported

Endotoxin

≤1EU/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Directions per Liter

Dissolve 8.85 grams in 900ml of ddH2O stirring gently until completely solubilized. DO NOT heat. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as

g/liter

Inorganic Salts

Calcium Chloride 2H2O
Cupric Sulfate•5H2O	0.0000013
Ferric Nitrate•9H2O	0.00005
Ferrous Sulfate•7H2O	0.000417
Magnesium Chloride	0.02866
Magnesium Sulfate	0.04884
Potassium Chloride	0.3118
Sodium Chloride	6.996
Sodium Phosphate dibasic	0.07102
Sodium Phosphate Monobasic	0.0543
Zinc Sulfate•7H2O	0.000432

Amino Acids

L-Alanine	0.00445
L-Arginine	0.12197
L-Asparagine•H2O	0.0075
L-Aspartic Acid	0.00665
L-Cysteine•HCl•H2O	0.01756
L-Cystine•2HCl	0.03129
L-Glutamic Acid	0.00735
L-Glutamine	0.365
Glycine	0.01875
L-Histidine HCl•H2O	0.03148
L-Isoleucine	0.05447
L-Leucine	0.05905
L-Lysine•HCl	0.09125
L-Methionine	0.01724
L-Phenylalanine	0.03548
L-Proline	0.01725
L-Serine	0.02625
L-Threonine	0.05345
L-Tryptophan	0.00902
L-Tyrosine•2Na•2H20	0.05579
L-Valine	0.05285

Vitamins

Biotin	0.0000035
Choline Chloride	0.00898
Vitamin B12	0.00068
Folic Acid	0.00266
i-inositol	0.0126
Nicotinamide	0.00202
D-Pantothenic Acid•Ca	0.00224
Pyridoxal Hydrochloride	0.002
Pyridoxine HCl	0.000031
Riboflavin	0.000219
Thiamine HCl	0.00217

Other

Thymidine	0.000365
D-Glucose Absent
Hypoxanthine	0.0021
Linoleic Acid	0.000042
Lipoic Acid	0.000105
Phenol Red•Na	0.00863
Putrescine•HCl	0.000081
Pyruvic Acid, Sodium	0.055

Total:

8.85g/Liter

References

1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9 (1974, May-Jun).

USBio References

1. Biondani G., et al. Extracellular matrix composition modulates PDAC parenchymal and stem cell plasticity and behavior through the secretome. FEBS J. 2018 Apr 16. doi: 10.1111/febs.14471.|2. Marengo A., et al. 2019. Pancreatic cancer stem cell proliferation is strongly inhibited by diethyldithiocarbamate-copper complex loaded into hyaluronic acid decorated liposomes. Biochim Biophys Acta Gen Subj. 1863(1):61-72. doi: 10.1016/j.bbagen.2018.09.018.

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D9807-02 Dulbecco’s MEM (DMEM) F-12 w/o Glucose

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